V(D)J recombinations in lymphocytes are essential for immunological diversity. They are also useful markers of pathologies, and in leukemia, are used to quantify the minimal residual disease during patient follow-up. High-throughput sequencing (NGS/HTS) now enables the deep sequencing of lymphoid populations. With dedicated Rep-Seq bioinformatics methods and software, clinicians and researchers can now qualify and sometimes quantify the heterogeneity of these lymphoid populations.
This fifth workshop is intended for users and developers of Vidjil, to share experience, protocols and results, to raise questions and decide development priorities, and possibly to set up new collaborations between biologists and bioinformaticians. The workshop will include invited talks, hematology, immunology and bioinformatics talks from the users and developers of Vidjil, and practical hands-on sessions on participant data. The Vidjil Workshop usually takes place every 18 months. The previous workshops (2016, 2017, 2019, special edition 2020) gathered at each edition between 30 and 40 scientists from 5 to 7 countries.
Hybrid participation.The 2022 edition comes backs physical, in Lille! However, it's also possible to follow remotely part of the workshop (hybrid): Zoom link, Meeting ID: 943 7024 7582, Passcode: 089129
The workshop includes invited talks, hematology and bioinformatics talks from the users and developers of Vidjil, and practical hands-on sessions on participant data. We expect that one participant from each group will do a short presentation of his research or clinical work, and, if applicable, of the results obtained with Vidjil or with other software.
For the practical sessions, the participants are invited to bring a laptop and some of their NGS data (fasta / fastq / fastq.gz files). The data can also be uploaded before the workshop into user accounts on app.vidjil.org before the workshop. We also provide public test data for the people who do not currently have their own NGS Rep-Seq data.
|9:00 – 9:45||Registration, coffee|
|9:45 – 10:00||hybrid Introduction|
|10:00 – 11:30|| hybrid Talks: Hematology and Immunology (session 1)
|11:30 – 12:30||
Talks: Algorithmics and bioinformatics
|12:30 – 14:00||Lunch|
|14:00 – 15:30||Practical sessions 1
|15:30 – 16:00||Coffee Break|
|16:00 – 17:00|| hybrid Keynote Talk
Nina Luning Park (U. Pennsylvania 🇺🇸) and Anne Eugster (TU Dresden 🇩🇪), AIRR Community: Biological Resources Working Group and challenges regarding IgH gene rearrangement analysis
|17:00 – 17:30|| hybrid
Talks: Bioinformatics software, regulation and certification
|20:00||Dinner, restaurant L'Assiette du Marché, in Lille.
800m from the Rihour metro station.
|9:00 – 10:30|| hybrid Talks: Hematology and Immunology (session 2)
|10:30 – 11:00||Coffee Break|
|11:00 – 12:30||Practical session 2
|12:30 – 14:00||Lunch|
|14:00 – 15:30|| Round table
|15:30 – 16:00||Conclusion
Discussion on library preparation, sequencing instruments and protocols, bioinformatics analysis. Conclusion on development priorities.
The workshop is sponsored by the VidjilNet consortium at Inria. There is no registration fee. To register, please copy, paste and fill the following lines into an email to Florian, Marc, Mathieu and Mikaël :
================================================ First name: Last name: Function: Laboratory/hospital: City: Country: I will be present in Lille: [ ] Both days [ ] Monday 16 May 2022 [ ] Tuesday 17 May 2022 [ ] I will attend the workshop remotely My usage of Vidjil: [ ] I use Vidjil at least once a month [ ] I use Vidjil occasionnaly [ ] I am a new user or I don't use Vidjil ================================================
The two lunches (16 and 17 May) and the dinner of the evening of the 17 May are free for registered participants. The registration for the full workshop with the lunches in the Lille is now closed, but stays open for the online participation.
The workshop takes place at CRIStAL ESPRIT building, in the heart of the Université de Lille Campus, at 20 minutes from Lille center. From Lille center, take the metro line 1 (yellow), direction 4 Cantons. Stop at the terminus, 4 Cantons -- Stade Pierre Mauroy station. The CRIStAL ESPRIT building is 300 meters from the metro station.
The workshop is organized by Marc Duez, Mathieu Giraud, Mikaël Salson, Florian Thonier, from CRIStAL, Inria Lille and the VidjilNet consortium.